Journal: The British journal of surgery
Article Title: A novel de novo synthesized phosphate carrier compound ABA-PEG20k-Pi20 suppresses collagenase production in Enterococcus faecalis and prevents colon anastomotic leak in mice
doi: 10.1002/bjs.10859
Figure Lengend Snippet: (A), Enterococcal- specific plate covered with skim milk plate to assess the total and collagenolytic counts of Enterococcus. The upper panel represents the E. faecalis group. The red arrow indicates collagenolytic colonies identified by the appearance of a halo surrounding the colonies. The bottom panel represents the E. faecalis + ABA-PEG20k-Pi20 group. The black arrow indicates non-collagenolytic colonies. (B), Total colony counts of Enterococcus. (C), Counts of collagenolytic strains. n=5/group, *p=0.0159, **p=0.0079 by nonparametric Mann-Whitney test. Dots represent bacterial counts in each mouse and long lines in between dots represent mean values of bacterial counts for each group.
Article Snippet: As mentioned, anastomotic tissues were homogenized, serially diluted in normal saline (0.9% NaCl), and 50 μl of each dilution was plated on Enterococcal selective plates (BD Difco).
Techniques: MANN-WHITNEY